Anti-CD4 Antibody [CC8] (A280065)

Anti-CD4 Antibody [CC8]

Mouse monoclonal [CC8] antibody to CD4.

A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324?T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori, et al. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori, et al. 2020).

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Bovine

Bovine lymphocytes.

Mouse

Monoclonal

CC8

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Bovine CD4 is the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4 antibody, clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4