Anti-CD4 Antibody [CC8] (PE) (A280064)

Anti-CD4 Antibody [CC8] (PE)

Mouse monoclonal [CC8] antibody to CD4 (PE).

A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324?T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori, et al. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori, et al. 2020).

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Bovine

Bovine lymphocytes.

Mouse

Monoclonal

CC8

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Bovine CD4 is the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4 antibody, clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

This product is for research use only. It is not intended for diagnostic or therapeutic use.