This antibody reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Rat
Immunogen
MLR generated rat Th cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-35
Isotype
IgG2a
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed) in flow cytometry surface staining of rat splenocyte suspension, labeling CD4 with Anti-CD4 Antibody [OX-35] (PE) (A86703), (concentration in sample 5 µg/ml).
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