This antibody recognises the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Dog
Immunogen
CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG.
Host
Mouse
Clonality
Monoclonal
Clone ID
LOB7/6
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions.
Figure A. Anti-CD19 Antibody [LT19] (FITC) (A280946) and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. Anti-CD19 Antibody [LT19] (FITC) (A280946) and Anti-CD40 Antibody [LOB7/6] (PE) (A280898). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .
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