This antibody recognises the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions.
Applications
IHC-P, IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 20 µg/ml, IHC-Fr: 1 µg/ml - 10 µg/ml, IHC-P: 1 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Dog
Immunogen
CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG.
Host
Mouse
Clonality
Monoclonal
Clone ID
LOB7/6
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions.
Immunofluorescence staining of a human tonsil cryosection with Anti-CD40 Antibody [LOB7/6] (A280900), red in A and Anti-CD21 Antibody [LB21] (A280858), green in B. The merged image is C with nuclei counterstained blue using DAPI. Low power
Immunofluorescence staining of a human tonsil cryosection with Anti-CD40 Antibody [LOB7/6] (A280900), red in A and Anti-CD21 Antibody [LB21] (A280858), green in B. The merged image is C with nuclei counterstained blue using DAPI. Medium power
Immunofluorescence staining of a human tonsil cryosection with Anti-CD40 Antibody [LOB7/6] (A280900), red in A and Anti-CD21 Antibody [LB21] (A280858), green in B. The merged image is C with nuclei counterstained blue using DAPI. High power
Recombinant humanized monoclonal antibody to CD40 for use as a research grade Ravagalimab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
Recombinant humanized monoclonal antibody to CD40 for use as a research grade Ravagalimab biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.