Recombinant rabbit monoclonal [HCAM/2875R] antibody to CD44 (CF®740).
Applications
IHC, IHC-P, WB
Positive Control
HeLa cells and paracortex in tonsil or lymph node tissue.
Dilutions
IHC-P: 0.25-0.5 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Stimulated human leukocytes.
Host
Rabbit
Clonality
Monoclonal
Clone ID
HCAM/2875R
Isotype
IgG
Conjugate
CF®740
Concentration
0.1 mg/mL
Predicted MW
80-95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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