This antibody recognises the porcine CD44 cell surface antigen which is involved in cell-cell interactions, cell adhesion and migration. CD44 is also a receptor for hyaluronic acid and can interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:200
Reactivity
Porcine, Dog, Horse, Bovine, Goat, Sheep
Immunogen
Purified porcine CD44.
Host
Rat
Clonality
Monoclonal
Clone ID
MAC329
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Pig CD44 antibody, clone MAC329 recognizes the porcine CD44 cell surface antigen which is involved in cell-cell interactions, cell adhesion and migration. CD44 is also a receptor for hyaluronic acid and can interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs).
Figure A. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Rat IgG1 - Isotype Control (A284009) detected with Goat F(ab')2 Anti-Rat IgG Antibody (PE), Cross-Adsorbed (A282657). Figure B. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Anti-CD44 Antibody [MAC329] (A281021) detected with Goat F(ab')2 Anti-Rat IgG Antibody (PE), Cross-Adsorbed (A282657). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.
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