Mouse monoclonal [MEM-85] antibody to CD44 (APC-Cyanine 7).
Specificity
This antibody reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen, a 80-95 kDa transmembrane glycoprotein of the hyaladherin family, present on the surface of most cells and tissues, including: leukocytes, endothelial cells, mesenchymal cells, etc. It is negative on platelets and hepatocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Leukocytes from a patient suffering from a LGL Type leukemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-85
Isotype
IgG2b
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
80-95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD44 Antibody [MEM-85] (APC-Cyanine 7) (4 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD44
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.