Anti-CD45 Antibody [2D1] (A85817)

Flow cytometry surface staining pattern of human peripheral whole blood labeling CD45 with Anti-CD45 Antibody [2D1] (A85817), (concentration in sample 3 µg/ml, GAM APC).

Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry surface staining of peripheral whole blood, labeling CD45 with Anti-CD45 Antibody [2D1] (A85817), (concentration in sample 3 µg/ml, GAM APC).

Anti-CD45 Antibody [2D1] (A85817) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody 2D1 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.