This antibody recognizes an extracellular epitope on all isoforms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except erythrocytes and platelets.
Applications
Flow Cytometry, IP, WB, IHC-P, IHC-Fr, ICC
Dilutions
WB: 1 µg/ml; Non-reducing conditions, band around 200-250 kDa; Positive Control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; Non-reducing conditions, 6% separating gel, Flow Cytometry: 1-4 µg/ml
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
HI30
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein G chromatography.
Concentration
1 mg/ml
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Anti-CD45 Antibody [HI30] (A85860) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI30 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
Publishing research using Anti-CD45 Antibody [HI30] (A85860)? Please let us know so that we can list the citation on this page.
![Flow Cytometry - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_1.jpg?profile=product_top)
![WB - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_1.jpg?profile=product_top_thumb)
![WB - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_1.jpg?profile=product_image)
![WB - Anti-CD45 Antibody [HI30] (A85860)](https://cdn.antibodies.com/image/catalog/85/A85860_2.jpg?profile=product_image)
![ICC - Anti-CD45 Antibody [MEM-28] (A85889)](https://cdn.antibodies.com/image/catalog/85/A85889_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-CD45 Antibody [2D1] (A85817)](https://cdn.antibodies.com/image/catalog/85/A85817_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [Bra55] - BSA and Azide free (A252962) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252962_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [2B11 + PD7/26] (A249809) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249809_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [PTPRC/1975R] - BSA and Azide free (A252997) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252997_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [2B11] (A249804) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249805_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [2B11 + PD7/26] - BSA and Azide free (A252989) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252989_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [rPTPRC/1460] (A249798) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249798_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [PTPRC/1975R] (A249817) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249817_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [rPTPRC/1460] - BSA and Azide free (A252978) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252978_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [SPM569 + SPM570] (A249809) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249810_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-CD45 Antibody [Bra55] (A249782) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249782_1.jpg?profile=product_alternative)