This antibody recognises a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5, also against CD45RC differing in their ability to stain B cells in tissue sections.
Applications
IHC-P, IHC-Fr, Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Porcine
Immunogen
Cells isolated from porcine mesenteric lymph node.
Host
Mouse
Clonality
Monoclonal
Clone ID
MIL15
Isotype
IgM
Conjugate
Unconjugated
Concentration
Lot Specific
Purity
Tissue Culture Supernatant.
Product Form
Liquid
Formulation
Supplied as tissue culture supernatant with 0.2M TRIS/HCl, pH 7.4, 5-10% foetal calf serum and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at 4°C.
General Notes
Mouse anti Pig CD45RC, clone MIL5 recognizes a restricted CD45 epitope encoded by the CD45 C exon and will bind and immunoprecipitate both the ~210 kDa RC and ~226 kDa RAC isoforms. MIL15, binds to an epitope alternative to that bound by clone MIL5, also against CD45RC differing in their ability to stain B cells in tissue sections (Zuckerman et al. 2001).
Figure A. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Mouse IgM - Isotype Control (A284020) detected with Goat Anti-Mouse IgM Antibody (PE). Figure B. Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589) and Anti-CD45RC Antibody [MIL15] (A281202) detected with Goat Anti-Mouse IgM Antibody (PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Figure A. Anti-CD45RC Antibody [MIL15] (A281202) detected with Goat Anti-Mouse IgM Antibody (PE) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD45RC Antibody [MIL15] (A281202) detected with Goat Anti-Mouse IgM Antibody (PE) and Anti-CD3 epsilon Antibody [PPT3] (FITC) (A281589). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
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