This antibody reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most thymocytes, and B lymphocytes subset (B-1a lymphocytes).
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD5 with Anti-CD5 Antibody [L17F12] (A86239), (concentration in sample 2 µg/ml, GAM APC).
Western blotting analysis of human CD5 using Anti-CD5 Antibody [L17F12] (A86239) on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.
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