This antibody reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).
Applications
Flow Cytometry, IP, WB, IHC-P, ELISA
Dilutions
ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA with the detection antibody CRIS1, IHC-P: 20 µg/ml; Positive Control: spleen, Flow Cytometry: 2 µg/ml, WB: Laurylmaltoside lysing buffer; Non-reducing conditions: 1-2 µg/ml.
Reactivity
Human
Immunogen
Crude thymus membrane fraction.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-32
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD5 with Anti-CD5 Antibody [MEM-32] (A86134), (concentration in sample 3 µg/ml, GAM APC).
Western blotting analysis of human CD5 using Anti-CD5 Antibody [MEM-32] (A86134) on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD5 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD5 was detected at approximately 62 kDa.
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