This antibody reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusively expressed on leukocytes. It is not present on platelets, red blood cells, and non-hematopoietic cells. This antibody also reacts with the deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Leukocytes from a patient suffering from a LGL Type leukemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-53
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
32-40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human monocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD53 with Anti-CD53 Antibody [MEM-53] (PE) (A85591), (20 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD53
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.