This antibody reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusively expressed on leukocytes. It is not present on platelets, red blood cells, and non-hematopoietic cells. This antibody also reacts with the deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Applications
Flow Cytometry, IP, WB, IHC-Fr
Dilutions
IHC-Fr: This antibody is suitable for discrimination of lymphomas from other tumors, Flow Cytometry: 1-5 µg/ml.
Reactivity
Human
Immunogen
Leukocytes from a patient suffering from a LGL Type leukemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-53
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
32-40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human monocytes (red-filled) from human CD53 negative blood debris (black-dashed) in flow cytometry surface staining of human peripheral blood, labeling CD53 with Anti-CD53 Antibody [MEM-53] (A85592), (concentration in sample 3 µg/ml, GAM APC).
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