This antibody recognises the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa.CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl or 100µl whole blood.
Reactivity
Human
Immunogen
TNF activated HUVEC cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
TEA3/18
Isotype
IgG1
Conjugate
Unconjugated
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD63 antibody, clone TEA3/18 recognizes the CD63 cell surface antigen, a glycoprotein of ~40-60 kDa.CD63 is expressed by monocytes and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation.
Synonyms
CD63 antigen, Granulophysin, LAMP-3, Limp1, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, MLA1, OMA81H, TSPAN30