This antibody, also known as Clone 34.1, recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
Applications
Flow Cytometry, IP, WB, IHC-Fr
Dilutions
Flow Cytometry: 1.5 µg/ml.
Reactivity
Rat
Immunogen
CD8 positive Wistar rat splenic T cell hybridomas.
Host
Mouse
Clonality
Monoclonal
Clone ID
341
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
32-34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of rat CD8b positive splenocytes (red-filled) from CD8b negative splenocytes (black-dashed) in flow cytometry surface staining of rat splenocyte suspension, labeling CD8 beta with Anti-CD8 beta Antibody [341] (A86238), (concentration in sample 1 µg/ml) GAM PE.
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