This antibody recognises the canine CD8ß cell surface antigen, expressed by cytotoxicsuppressor T lymphocytes that interact with MHC Class I expressing targets.
Applications
IHC-Fr, IP, Flow Cytometry
Dilutions
Flow Cytometry: Neat, IHC-Fr 1: 1:10, Use 20µl of the suggested working dilution to label 106 cells or 100µl whole blood
Reactivity
Canine
Host
Mouse
Clonality
Monoclonal
Clone ID
CA15.4G2
Isotype
IgG1
Conjugate
Unconjugated
Concentration
Lot Specific
Purity
Tissue Culture Supernatant
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Dog CD8 beta antibody, clone CA15.4G2 recognises the canine CD8ß cell surface antigen, expressed by cytotoxicsuppressor T lymphocytes that interact with MHC Class I expressing targets.
Figure A. A488 conjugated Mouse anti Canine CD4 and Mouse IgG1 - Isotype Control (A284021) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. A488 conjugated Mouse anti Canine CD4 and Anti-CD8 beta Antibody [CA15.4G2] (A279973) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. Anti-CD8 beta Antibody [CA15.4G2] (A279973) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b (FITC) - Isotype Control (A282450). Figure B. Anti-CD8 beta Antibody [CA15.4G2] (A279973) detected with Goat anti Mouse IgG1 DyLight 649 and Anti-CD4 Antibody [YKIX302.9] (FITC) (A282041). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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