This antibody reacts with an extracellular epitope of CD31, a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells, and stem cells of the myeloid lineage.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Rabbit, Feline, Human
Immunogen
MOLT-4 (human T-ALL cell line).
Host
Mouse
Clonality
Monoclonal
Clone ID
M38
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
130-140 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
26 kDa cell surface protein TAPA-1, CD81 antigen, TAPA1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, TSPAN28
Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD81 with Anti-CD81 Antibody [M38] (PE) (A86718), (20 µl reagent / 100 µl of peripheral whole blood).
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