This antibody reacts with an extracellular epitope of CD31, a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells, and stem cells of the myeloid lineage.
FUNC: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines, Flow Cytometry: 1 µg/ml, WB: 1-2 µg/ml; Positive Control: Jurkat cells, Non-reducing conditions.
Reactivity
Rabbit, Feline, Human
Immunogen
MOLT-4 (human T-ALL cell line).
Host
Mouse
Clonality
Monoclonal
Clone ID
M38
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
130-140 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
26 kDa cell surface protein TAPA-1, CD81 antigen, TAPA1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, TSPAN28
Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD81 with Anti-CD81 Antibody [M38] - Low endotoxin, Azide free (A86864), (low endotoxin, concentration in sample 4 µg/ml) GAM APC.
Western blotting analysis of human CD81 using Anti-CD81 Antibody [M38] - Low endotoxin, Azide free (A86864) on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.
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