This antibody recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PHA-activated peripheral blood cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-180
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Purified antibody is conjugated with PE under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
ADGRE5, Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD97 with Anti-CD97 Antibody [MEM-180] (PE) (A320666), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD97
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.