Rabbit polyclonal antibody to Cdc25B (Phospho-Ser149)
Specificity
The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Predicted MW
62kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149) Antibody #11553.
Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody #11553.
Publishing research using Anti-Cdc25B (Phospho-Ser149) Antibody (A50882)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Cdc25B (Phospho-Ser149) Antibody (A50882)
![Western Blot - Anti-Cdc25B Antibody [ARC1736] (A308797) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308797_1.jpg?profile=product_alternative)
