Rabbit polyclonal antibody to CDC25B (Phospho-Ser323)
Specificity
The antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody #12104. The lane on the right is treated with the synthesized peptide.
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