Anti-CENPA (phospho Ser18) Antibody (A320105)

Anti-CENPA (phospho Ser18) Antibody

Rabbit polyclonal antibody to CENPA (phospho Ser18).

This antibody recognises centromere protein A when phosphorylated at serine 18.CENP-A is a histone H3 variant which replaces one or both canonical H3 histones in a subset of nucleosomes within centromeric chromatin and epigenetically determines the position of the centromere on each chromosome, and where kinetochore assembly and sister chromatid cohesion occurs during mitosis. CENP-A has been shown to interact directly with the inner kinetochore through CENP-C and CENP-N. This interaction allows microtubules to accurately segregate chromosomes during mitosis. Phosphorylation of CENP-A on serine 18 is thought to play an important role in the segregation of chromosomes during mitosis. Rabbit anti human CENP-A (pSer18) antibody shows minimal cross-reactivity with CENP-A that has been phosphorylated at serine 16.

IF, WB

IF: 1:100, WB: 1:100 - 1:500

Human

Synthetic peptide of phosphorylated serine 16 and 18 of human CENP-A.

Rabbit

Polyclonal

IgG

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti human CENP-A (pSer18) antibody recognizes centromere protein A when phosphorylated at serine 18.CENP-A is a histone H3 variant which replaces one or both canonical H3 histones in a subset of nucleosomes within centromeric chromatin and epigenetically determines the position of the centromere on each chromosome, and where kinetochore assembly and sister chromatid cohesion occurs during mitosis (Fachinetti et al. 2013) (Blower et al. 2002). CENP-A has been shown to interact directly with the inner kinetochore through CENP-C and CENP-N. This interaction allows microtubules to accurately segregate chromosomes during mitosis (Kixomoeller et al. 2020) (Yan et al. 2019). Phosphorylation of CENP-A on serine 18 is thought to play an important role in the segregation of chromosomes during mitosis (Kixomoeller et al. 2020) (Zhang et al. 2017).Rabbit anti human CENP-A (pSer18) antibody shows minimal cross-reactivity with CENP-A that has been phosphorylated at serine 16.

CENP-A, Centromere autoantigen A, Centromere protein A, Histone H3-like centromeric protein A