Anti-CK1 epsilon Antibody (A83740)

Name: Anti-CK1 epsilon Antibody
Brand: Antibodies.com
SKU: A83740
Price: 115.0 GBP
Availability: InStock

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$495

Goat polyclonal antibody to CK1 epsilon for ELISA, WB, IHC, IF and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CK1 epsilon Antibody

Description

Goat polyclonal antibody to CK1 epsilon.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.3-1µg/ml, IHC: 5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human CK1 epsilon.

Sequence

C-PASQTSVPFDHLGK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

48 kDa

Predicted MW

47.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Casein kinase 1 epsilon, Casein kinase I, Casein kinase I epsilon, Casein kinase I isoform epsilon, CKI epsilon, CKI-epsilon, CKIe, CKIepsilon, CSNK 1E, CSNK1E, DBT, Doubletime, Drosophila, homolog of, EC 2.7.11.1, epsilon isoform, HCK IE, KC1E, KC1E_HUMAN, kinase CK1 epsilon, MGC 10398

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-CK1 epsilon Antibody (A83740)

CK1 epsilon expression in A431 (A), HeLa (B) and K562 (C) nuclear cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CK1 epsilon Antibody (A83740) at 1µg/ml and detected by chemiluminescence.

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Immunofluorescence - Anti-CK1 epsilon Antibody (A83740)

CK1 epsilon expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CK1 epsilon Antibody (A83740) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Immunofluorescence - Anti-CK1 epsilon Antibody (A83740)

CK1 epsilon expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CK1 epsilon Antibody (A83740) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Flow Cytometry - Anti-CK1 epsilon Antibody (A83740)

CK1 epsilon expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-CK1 epsilon Antibody (A83740) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.