Anti-Collagen III Antibody (A14465)

Name: Anti-Collagen III Antibody
Brand: Antibodies.com
SKU: A14465
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Collagen III for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Collagen III Antibody

Description

Rabbit polyclonal antibody to Collagen III.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1217-1466 of human Collagen III alpha 1/COL3A1 (NP_000081.1).

Sequence

APYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

COL3A1, Collagen alpha-1(III) chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Collagen III Antibody (A14465)

Western blot analysis of extracts of Mouse brain, using Anti-Collagen III Antibody (A14465) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-Collagen III Antibody (A14465)

Immunofluorescence analysis of RD cells using Anti-Collagen III Antibody (A14465) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Collagen III Antibody (A14465)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Collagen III Antibody (A14465) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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