Rabbit polyclonal antibody to Connexin 43 (Phospho-Tyr265)
Specificity
Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Tyrosine 265
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 265(Q-K-Y(p)-A-Y) derived from Human Connexin 43.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Connexin-43, Cx43, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein, GJA1, GJAL
Western blot analysis of extracts from HuvEc cells using Connexin 43 (Phospho-Tyr265) Antibody #11767.The lane on the right is treated with the antigen-specific peptide.
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