Anti-CoREST Antibody (A90688)

Name: Anti-CoREST Antibody
Brand: Antibodies.com
SKU: A90688
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to CoREST for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CoREST Antibody

Description

Rabbit polyclonal antibody to CoREST.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 256-485 of human CoREST/CoREST/RCOR1 (NP_055971.2).

Sequence

EREESEDELEEANGNNPIDIEVDQNKESKKEVPPTETVPQVKKEKHSTQAKNRAKRKPPKGMFLSQEDVEAVSANATAATTVLRQLDMELVSVKRQIQNIKQTNSALKEKLDGGIEPYRLPEVIQKCNARWTTEEQLLAVQAIRKYGRDFQAISDVIGNKSVVQVKNFFVNYRRRFNIDEVLQEWEAEHGKEETNGPSNQKPVKSPDNSIKMPEEEDEAPVLDVRYASAS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

KIAA0071, Protein CoREST, RCOR, RCOR1, REST corepressor 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CoREST Antibody (A90688)

Western blot analysis of extracts of various cell lines, using Anti-CoREST Antibody (A90688) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-CoREST Antibody (A90688)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-CoREST Antibody (A90688). This Western blot was performed on the immunoprecipitate using Anti-CoREST Antibody (A90688) at a dilution of 1:1000.

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Chromatin Immunoprecipitation - Anti-CoREST Antibody (A90688)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HepG2 cells, using Anti-CoREST Antibody (A90688) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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