Rabbit polyclonal antibody to CRMP-2 (Phospho-Thr509)
Specificity
The antibody detects endogenous levels of CRMP-2 only when phosphorylated at threonine 509.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 509 (S-V-T(p)-P-K) derived from Human CRMP-2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DPYSL2, DRP-2, N2A3, ULIP-2, ULIP2, Unc-33-like phosphoprotein 2
Western blot analysis of extracts from HT-29 cells treated with heat shock using CRMP-2 (Phospho-Thr509) Antibody #11795.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CRMP-2 (Phospho-Thr509) antibody #11795 (left)or the same antibody preincubated with blocking peptide (right).
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