Rabbit polyclonal antibody to CXCR4 (phospho Ser346 + Ser347).
Specificity
This antibody targets the phosphorylated form of human CXCR4 at residues Ser346 and Ser347. It detects agonist-induced phosphorylation in Western blot analysis, including after PKC activation.
Applications
WB
Dilutions
WB: 1:1,000
Reactivity
Human, Mouse
Immunogen
Synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser346 + Ser347. Immunogen range is 10-12 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Analysis of agonist-induced phosphorylation of CXC Chemokine Receptor 4 (CXCR4) at Serine346/Serine347. HEK293 cells stably expressing HA-tagged CXCR4 were untreated or treated with 100 nM CXCL12 for 30 min. Cell lysates were immunoblotted with anti-CXCR4 (Phospho Ser346 + Ser347) antibody (A334500) at 1:1000 (upper panel). The blot was stripped and reprobed with anti-HA antibody at 1:1000 to verify equal gel loading (lower panel).
Dose-dependent phosphorylation of CXC Chemokine Receptor 4 (CXCR4) at Serine346/Serine347. HEK293 cells stably expressing HA-tagged CXCR4 were untreated or exposed to 1 nM to 100 nM CXCL12 for 30 min. Lysates were immunoblotted with anti-CXCR4 (Phospho Ser346 + Ser347) antibody (A334500) at 1:1000 (upper panel), stripped and reprobed with phosphorylation-sensitive anti-CXCR4 antibody (A334501) at 1:1000 (middle panel), and stripped again and reprobed with anti-HA antibody at 1:1000 for equal loading (lower panel).
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![SDS-PAGE - Anti-CXCR4 Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A323988) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323988_1.jpg?profile=product_alternative)
