Western blot analysis of extracts of HepG2 cells, using Anti-Cyclin B1 Antibody (A8393). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
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