Anti-Cyclin D1 Antibody [CCND1/809] (Biotin) (A352886)

Anti-Cyclin D1 Antibody [CCND1/809] (Biotin)

Mouse monoclonal [CCND1/809] antibody to Cyclin D1 (Biotin).

Flow Cytometry (Intracellular), IHC, IHC-P

T47D, ZR-75, BT-474, SK-BR-3, MCF-7, MDA-MB-453, HT-29, Ramos, Jurkat, and A431 cells; mantle cell lymphoma, breast carcinoma, or bladder carcinoma tissue.

Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Human

Recombinant human Cyclin D1 (CCND1) protein.

Mouse

Monoclonal

CCND1/809

kappa

0.1 mg/mL

36 kDa

Liquid

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1, CCND1, G1/S-specific cyclin-D1, PRAD1, PRAD1 oncogene

This product is for research use only. It is not intended for diagnostic or therapeutic use.