Mouse monoclonal [CCND1/809] antibody to Cyclin D1 (CF®647).
Applications
Flow Cytometry (Intracellular), IHC, IHC-P
Positive Control
T47D, ZR-75, BT-474, SK-BR-3, MCF-7, MDA-MB-453, HT-29, Ramos, Jurkat, and A431 cells; mantle cell lymphoma, breast carcinoma, or bladder carcinoma tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant human Cyclin D1 (CCND1) protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
CCND1/809
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®647
Excitation: 650nm, Emission: 665nm
Concentration
0.1 mg/mL
Predicted MW
36 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
