Mouse monoclonal [DCS-6] antibody to Cyclin D1 (CF®740).
Applications
Flow Cytometry (Intracellular), IHC, IHC-P, WB
Positive Control
T47D, ZR-75, BT-474, SK-BR-3, MCF-7, MDA-MB-453, HT-29, Ramos, Jurkat, and A431 cells; mantle cell lymphoma, breast carcinoma, or bladder carcinoma tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL, WB: 0.5-1 µg/mL, IHC-P: 0.5-1 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human, Monkey, Mouse, Rat
Immunogen
Recombinant full-length human Cyclin D1 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
DCS-6
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®740
Concentration
0.1 mg/mL
Predicted MW
36 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
