A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human Cytokeratin 14 (Cytokeratin 14 (KRT14)) (NP_000517.2).
Western Blot - Anti-Cytokeratin 14 Antibody (A90527)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 14 Antibody (A90527) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat skin using Anti-Cytokeratin 14 Antibody (A90527) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse skin using Anti-Cytokeratin 14 Antibody (A90527) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-431 cells using Anti-Cytokeratin 14 Antibody (A90527) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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