Mouse monoclonal [C-04] antibody to Cytokeratin 18.
Specificity
This antibody reacts with Cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
WB: 1-2 µg/ml, IHC-P: 10 µg/ml; Positive Control: colon, kidney, ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100), Flow Cytometry: 1-4 µg/ml; Intracellular staining.
Reactivity
Mammalian
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Host
Mouse
Clonality
Monoclonal
Clone ID
C-04
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cell proliferation-inducing gene 46 protein, CK-18, CYK18, Cytokeratin-18, K18, Keratin, type I cytoskeletal 18, Keratin-18, KRT18
Separation of HeLa cells stained with Anti-Cytokeratin 18 Antibody [C-04] (A86656), (concentration in sample 0,6 µg/ml, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.
Western blotting analysis of human cytokeratin 18 using Anti-Cytokeratin 18 Antibody [C-04] (A86656) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 46 kDa.
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