WB: 1-2 µg/ml, IHC-P: 10 µg/ml; Positive Control: colon, skin, IHC: Positive Control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line, ELISA: Positive Control: MCF-7 human breast adenocarcinoma cell line; This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100), Flow Cytometry: 1-4 µg/ml; Intracellular staining.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Dog, Mouse, Rat, Pig, Hamster, Sheep or Cow.
Immunogen
Human breast carcinoma cell line PMC-42.
Host
Mouse
Clonality
Monoclonal
Clone ID
DC-10
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cell proliferation-inducing gene 46 protein, CK-18, CYK18, Cytokeratin-18, K18, Keratin, type I cytoskeletal 18, Keratin-18, KRT18
Western blotting analysis of human cytokeratin 18 using Anti-Cytokeratin 18 Antibody [DC-10] (A86578) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.
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