Anti-Cytokeratin 18 Antibody [DC-10] (Biotin) (A86580)

Name: Anti-Cytokeratin 18 Antibody [DC-10] (Biotin)
Brand: Antibodies.com
SKU: A86580
Price: 125.0 GBP
Availability: InStock

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$210

Mouse monoclonal [DC-10] antibody to Cytokeratin 18 (Biotin) for IP, WB, IHC-P, ICC, ELISA and Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

FITC Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Epithelial Cell Marker Hepatocyte Marker

Specifications

Name

Anti-Cytokeratin 18 Antibody [DC-10] (Biotin)

Description

Mouse monoclonal [DC-10] antibody to Cytokeratin 18 (Biotin).

Specificity

This antibody reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Applications

IP, WB, IHC-P, ICC, ELISA, Flow Cytometry

Dilutions

Flow Cytometry: 2-6 µg/ml; Intracellular staining, WB: 1-2 µg/ml.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Dog, Mouse, Rat, Pig, Hamster, Sheep or Cow.

Immunogen

Human breast carcinoma cell line PMC-42.

Host

Mouse

Clonality

Monoclonal

Clone ID

DC-10

Isotype

IgG1

Conjugate

Biotin

Purification

This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.

Concentration

1 mg/ml

Predicted MW

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Synonyms

Cell proliferation-inducing gene 46 protein, CK-18, CYK18, Cytokeratin-18, K18, Keratin, type I cytoskeletal 18, Keratin-18, KRT18

Isotype Controls

Mouse IgG1 [MOPC-21] (Biotin) (A86204)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-Cytokeratin 18 Antibody [DC-10] (Biotin) (A86580)

Flow cytometric surface staining of HeLa cells labeling Cytokeratin 18 with Anti-Cytokeratin 18 Antibody [DC-10] (Biotin) (A86580) concentration in sample 6 µg/ml) streptavidin-APC, with blank sample (black).

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Anti-Cytokeratin 18 Antibody [DC-10] (Biotin) (A86580)

Western blotting analysis of human cytokeratin 18 using Anti-Cytokeratin 18 Antibody [DC-10] (Biotin) (A86580) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.

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