Western Blot - Anti-Cytokeratin 8 (phospho Ser74) Antibody (A329308)
Cytokeratin 8 expression in HepG2 cell lysates analyzed by western blot. Cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Cytokeratin 8 (Phospho S74) Antibody (A329308) at a dilution of 1:1000 and detected with chemiluminescence.
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