Rabbit polyclonal antibody to DAPP1 (phospho Tyr139).
Specificity
This antibody detects endogenous levels of DAPP1 only when phosphorylated at Tyr139.
Applications
WB, IF, ELISA
Dilutions
IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human DAPP1 around the phosphorylation site of Tyr139 (amino acids 105-154).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
32kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B lymphocyte adapter protein Bam32, B-cell adapter molecule of 32 kDa, BAM32, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1
Western Blot - Anti-DAPP1 (phospho Tyr139) Antibody (A93866)
Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 2' using Anti-DAPP1 (phospho Tyr139) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence analysis of A549 cells using Anti-DAPP1 (phospho Tyr139) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
