Western Blot - Anti-DDX3X Antibody [Assigned #A10099] (A11240)
Western blot analysis of extracts of various cell lines, using Anti-DDX3X Antibody (A10099) at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.