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Anti-DIAPH1 Antibody (A14967)

Western Blot - Anti-DIAPH1 Antibody (A5772) - Antibodies.com Immunofluorescence - Anti-DIAPH1 Antibody (A5772) - Antibodies.com
This product is discontinued

Anti-DIAPH1 Antibody (A14967) has been discontinued and is no longer available.

View all DIAPH1 Antibodies.

  • Specifications

Name
Anti-DIAPH1 Antibody
Description
Rabbit polyclonal antibody to DIAPH1.
Applications
WB, IF
Dilutions
WB: 1:500-1:2000, IF: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1149-1263 of human DIAPH1 (NP_001073280.1).
Sequence
TEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
178kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DIAP1, Diaphanous-related formin-1, DRF1, Protein diaphanous homolog 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-DIAPH1 Antibody (A5772) - Antibodies.comEnlarge Image

Western Blot - Anti-DIAPH1 Antibody (A14967)

Western blot analysis of extracts of various cell lines, using Anti-DIAPH1 Antibody (A5772) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunofluorescence - Anti-DIAPH1 Antibody (A5772) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-DIAPH1 Antibody (A14967)

Immunofluorescence analysis of U2OS cells using Anti-DIAPH1 Antibody (A5772). Blue: DAPI for nuclear staining.

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Proteins predicted to interact with DIAPH1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.4% Relevancy Score
97.3% Relevancy Score
97.2% Relevancy Score
97.1% Relevancy Score
97% Relevancy Score
95.5% Relevancy Score
93.6% Relevancy Score
93.4% Relevancy Score
93% Relevancy Score