Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CARP-1, CARP1, CCAR1, Cell cycle and apoptosis regulatory protein 1, Cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain
Western blot analysis of extracts of Mouse testis, using Anti-DIS Antibody (A15179) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.
Western blot analysis of extracts of HepG2 cells, using Anti-DIS Antibody (A15179) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of Rat spleen, using Anti-DIS Antibody (A15179) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-DIS Antibody (A15179). This Western blot was performed on the immunoprecipitate using Anti-DIS Antibody (A15179) at a dilution of 1:500.
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