Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CARP-1, CARP1, CCAR1, Cell cycle and apoptosis regulatory protein 1, Cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain
Western blot analysis of extracts of various cell lines, using Anti-DIS Antibody (A88366) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-DIS Antibody (A88366). This Western blot was performed on the immunoprecipitate using Anti-DIS Antibody (A88366) at a dilution of 1:500.
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