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Anti-E2F1 Antibody [Assigned #A2442] (A16827)

Western Blot - Anti-E2F1 Antibody (A2442) - Antibodies.com
This product is discontinued

Anti-E2F1 Antibody [Assigned #A2442] (A16827) has been discontinued and is no longer available.

View all E2F1 Antibodies.

  • Specifications

Name
Anti-E2F1 Antibody [Assigned #A2442]
Description
Mouse monoclonal (#A2442) antibody to E2F1.
Applications
WB, IF, IP
Dilutions
WB: 1:200-1:1000, IF: 1:50-1:100, IP: 1:20-1:50
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human E2F1.
Host
Mouse
Clonality
Monoclonal
Clone ID
Assigned #A2442
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
70kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
E2F-1, PBR3, pRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-E2F1 Antibody (A2442) - Antibodies.comEnlarge Image

Western Blot - Anti-E2F1 Antibody [Assigned #A2442] (A16827)

Western blot analysis of extracts of various cell lines, using Anti-E2F1 Antibody (A2442) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Proteins predicted to interact with E2F1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.5% Relevancy Score
99.4% Relevancy Score