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Anti-EGF Antibody - BSA and Azide free (A282983)

This product is discontinued

Anti-EGF Antibody - BSA and Azide free (A282983) has been discontinued and is no longer available.

View all EGF Antibodies.

Name
Anti-EGF Antibody - BSA and Azide free
Description
Rabbit polyclonal antibody to EGF.
Specificity
This antibody recognises rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
Applications
WB, ELISA, Functional Assays
Dilutions
ELISA: 0.5 - 2 µg/ml, Functional Assays: 0.05 - 0.1 µg/ml, WB: 0.5 - 1 µg/ml
Reactivity
Rat
Immunogen
Recombinant rat EGF.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
1 mg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Rat EGF antibody recognizes rat EGF (Epidermal Growth Factor), a type I transmembrane protein expressed by a variety of cells, which acts as a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, and is a key factor in cell survival.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the EGF receptor (EGFR), which results in the triggering of several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
Synonyms
Pro-epidermal growth factor
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

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