Mouse monoclonal [H9B4] antibody to EGFR (Biotin).
Applications
WB
Positive Control
A431 cells and breast or bladder carcinoma tissue.
Dilutions
WB: 0.5-1 µg/mL, IP: 1-2 µg/500 µg protein lysate. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Cross Reactivity
No cross-reactivity with Mouse or Rat. Cross-reactivity with other species has not been determined.
Immunogen
Purified EGFR from A431 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
H9B4
Isotype
IgG1
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
~170 kDa (wild type) and ~145 kDa (vIII variant)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
