Anti-eNOS (phospho Ser1177) Antibody (A16643)

Western blot analysis of extracts of Jurkat cells, using Anti-eNOS (phospho Ser1177) Antibody (A16643). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

Western blot analysis of extracts of HeLa cells, using Anti-eNOS (phospho Ser1177) Antibody (A16643) at 1:1,000 dilution or eNOS antibody (A1548). HeLa cells were treated by IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.