Western Blot - Anti-Eph receptor A2 (phospho Tyr772) Antibody (A88080)
Western blot analysis of extracts of C6 cells, using Anti-Eph receptor A2 (phospho Tyr772) Antibody (A88080) at 1:1,000 dilution. C6 cells were treated by EphrinA1 (100 ng/ml) for 5 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
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