Rabbit polyclonal antibody to EPHA4 (Phospho-Tyr596)
Specificity
The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y(p)-V-D) derived from Human EPHA4 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from JK cells (Lane 2), using EPHA4 (Phospho-Tyr596) Antibody #11834. The lane on the left is treated with antigen-specific peptide.
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